A method of extracting residual pesticides from livestock meat fat and a kit to be used therefor. Namely, a method of extracting residual pesticides comprising; (1) a step of homogenizing livestock meat fat, which is a solidified state, in the presence of water and then separating the fat, (2) a step of treating the thus treated fat with a dehydrating agent, and (3) a step of dissolving the dehydrated fat in a solvent in which fat is soluble, re-dissolving the pesticides in a hydrophilic solvent and then concentrating the solution of the hydrophilic solvent to give a liquid sample for measuring the residual pesticides. A kit to be used in the above-described method. Use of the above method and kit makes it possible to simplify the procedure while achieving an effect of reducing the amount of impurities (an organic acid, etc) extracted.