An apparatus and method for detecting a signal in a communication system using multiple antennas are provided. The apparatus includes an optimal orderer for determining an order of signals that are subject to detection, a controller for controlling a parallel successive interference canceller so as to successively detect the signals according to the determined signal detection order, cancel the successively detected signals from a received signal, detect the signals in reverse order of the signal detection order, and cancel the successively detected signals from the received signal, and outputting a last detected signal and the parallel successive interference canceller for successively canceling the detected signals from the received signal according to a control of the controller. Accordingly, the invention provides a signal detection apparatus and method that improve the reliability while reducing the complexity of a communication system using multiple antennas.