A stress testing method of a file system includes traversing local or network storage devices with a drive letter; detecting a network mapping path of the network storage devices; calculating an absolute path of all the storage devices through a mounted point and a system volume; collecting the above information to update the path information of the file system; and then calling a corresponding test algorithm and stressing strategy according to different types of storage devices, so as to perform the stress test. The stress testing method can make the file system display storage devices without a drive letter, and call appropriate testing methods and stressing strategies for different types of storage devices, so the depth and scope of the stress testing for file system are expanded, the accuracy of the test is enhanced, and the problem of occupying too many system resources is avoided.