发明申请
- 专利标题: NUCLEIC ACID AMPLIFICATION METHOD
- 专利标题(中): 核酸扩增方法
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申请号: US12191749申请日: 2008-08-14
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公开(公告)号: US20090155856A1公开(公告)日: 2009-06-18
- 发明人: Hayato MIYOSHI , Yoshihide Iwaki , Toshihiro Mori
- 申请人: Hayato MIYOSHI , Yoshihide Iwaki , Toshihiro Mori
- 优先权: JP2007-211693 20070815
- 主分类号: C12P19/34
- IPC分类号: C12P19/34
摘要:
An object to be achieved by the present invention is to provide a nucleic acid amplification method by which a nucleic acid can be amplified using oligonucleotide primers and DNA polymerase. The present invention provides a nucleic acid amplification method which comprises performing incubation of a reaction solution containing at least one type of deoxynucleotide triphosphate, at least one type of DNA polymerase having strand displacement activity, at least two types of oligonucleotide primer, and the nucleic acid fragment as a template so as to perform a polymerase reaction that initiates from the 3′ end of the primer and thus amplifying the nucleic acid fragment, wherein a first oligonucleotide primer and a second oligonucleotide primer are designed in such a way that a region which contains two identical sequences X of serial 4 or more nucleotides within, the region of 200 or less nucleotides, or apart thereof can be amplified.
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