发明申请
- 专利标题: Multi Plasmid System For The Production Of Influenza Virus
- 专利标题(中): 用于生产流感病毒的多质粒体系
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申请号: US12254131申请日: 2008-10-20
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公开(公告)号: US20090175907A1公开(公告)日: 2009-07-09
- 发明人: Erich Hoffman , Hong Jin , Bin Lu , Gregory Duke , George Kemble , Zhongying Chen
- 申请人: Erich Hoffman , Hong Jin , Bin Lu , Gregory Duke , George Kemble , Zhongying Chen
- 申请人地址: US MD Gaithersburg
- 专利权人: MedImmune, LLC
- 当前专利权人: MedImmune, LLC
- 当前专利权人地址: US MD Gaithersburg
- 主分类号: A61K39/145
- IPC分类号: A61K39/145 ; C12N7/01 ; C12N15/74 ; A61P31/16
摘要:
Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. A method of producing a cold adapted (ca) influenza virus that replicates efficiently at, e.g., 25° C. (and immunogenic compositions comprising the same) is also provided.