A TV tuner card operation method is described. A TV tuner module is provided to a computer, wherein the TV tuner module is electrically connected to the computer via a PCI-E interface. A remote controller is used to turn on an operation system of the computer via a wake pin of the PCI-E interface. The remote controller is used to operate all functions of the TV tuner module to display TV programs on a screen of the computer. When the PCI-E interface is adapted to a PCI-E card, the wake pin is B11 of the PCI-E card. When the PCI-E interface is adapted to a Mini card, the wake pin is Pin 1 of the Mini card. When the PCI-E interface is adapted to a PCI Express Card, the wake pin is Pin 11 of the PCI Express Card.