An automatic inspection system and method for a notebook computer keyboard interface are described. The method includes the following steps including scanning a notebook computer keyboard array to obtain a keyboard position corresponding table; running a testing software in the notebook computer to obtain a position value of a key under test from the keyboard position corresponding table; receiving a testing command from the testing software by an USB interface; constituting a virtual keyboard matrix with at least one matrix switching chip, for controlling the close action of a corresponding key position in the virtual keyboard matrix according to the testing command; transferring the actual key position value corresponding to the output close action to the notebook computer according to the configuration of the notebook computer keyboard interface; and receiving and comparing the actual key position value with the position value of the key under test.