A chassis structure with interface card bracket is described, which uses a bracket to assemble an interface card in an accommodation space within a chassis. The bracket includes a carrying member and an operating member that are pivoted to each other, and the interface card is fixed on the carrying member. The chassis includes a guide portion disposed therein. As the bracket moves into the accommodation space, the carrying member and the operating member are pivoted with respect to each other upon being guided by the guide portion to form an appropriate included angle there-between, so as to avoid barriers around the accommodation space, thereby assembling the interface card into the accommodation space successfully.