发明申请
US20090226959A1 Production of modified glycoproteins having multiple antennary structures
审中-公开
具有多个触角结构的修饰的糖蛋白的生产
- 专利标题: Production of modified glycoproteins having multiple antennary structures
- 专利标题(中): 具有多个触角结构的修饰的糖蛋白的生产
-
申请号: US12313636申请日: 2008-11-21
-
公开(公告)号: US20090226959A1公开(公告)日: 2009-09-10
- 发明人: Piotr Bobrowicz , Stephen R. Hamilton , Tillman U. Gerngross , Stefan Wildt , Byung-Kwon Choi , Juergen Hermann Nett , Robert C. Davidson
- 申请人: Piotr Bobrowicz , Stephen R. Hamilton , Tillman U. Gerngross , Stefan Wildt , Byung-Kwon Choi , Juergen Hermann Nett , Robert C. Davidson
- 主分类号: C12P21/00
- IPC分类号: C12P21/00 ; C12N1/19 ; C12N1/15 ; C12N15/63 ; C07K2/00
摘要:
The present invention relates to eukaryotic host cells, especially lower eukaryotic host cells, having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar and sugar nucleotide transporters to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells exhibit GnTIII, GnTIV, GnTV, GnT VI or GnTIX activity, which produce bisected and/or multiantennary N-glycan structures and may be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar, sugar nucleotide transporters, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.