A barcode label retention device is provided for a barcode printer. The barcode label retention device includes s at least one pair of label roll retainers, at least one pair of resilient elements, and at least one pair of fixing plates. The label roll retainers are mounted inside a housing of a barcode printer and spaced from each other to receive and retain a label roll therebetween. The resilient elements are arranged inside each label roll retainer, and the fixing plates are arranged inside each label roll retainer. The fixing plate is connected to the resilient element to provide the fixing plate with resiliency for inward depressibility or outward biasing so that a front portion of the fixing plate projects inwards beyond the label roll retainer to abut against opposite ends of the label roll, whereby the label roll can be securely held by the two fixing plates engaging the opposite ends thereof and the path of a winding/unwinding operation can be stabilized.