发明申请
US20090305385A1 Novel Cultured Silkworm Cells Capable of Highly Efficient Baculovirus Production and Protein Production
有权
新型培养的蚕细胞能够高效率的杆状病毒生产和蛋白质生产
- 专利标题： Novel Cultured Silkworm Cells Capable of Highly Efficient Baculovirus Production and Protein Production
- 专利标题（中）： 新型培养的蚕细胞能够高效率的杆状病毒生产和蛋白质生产
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申请号： US12226689申请日： 2007-04-26
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公开(公告)号： US20090305385A1公开(公告)日： 2009-12-10
- 发明人: Takahiro Kusakabe , Chisa Aoki , Osamu Ninagi , Jae Man Lee , Kazuhiro Iiyama , Naoya Kawakami
- 申请人： Takahiro Kusakabe , Chisa Aoki , Osamu Ninagi , Jae Man Lee , Kazuhiro Iiyama , Naoya Kawakami
- 申请人地址： JP Fukuoka-shi, Fukuoka
- 专利权人： Kyushu University, National University Corporation
- 当前专利权人： Kyushu University, National University Corporation
- 当前专利权人地址： JP Fukuoka-shi, Fukuoka
- 优先权： JP2006-122943 20060427
- 国际申请： PCT/JP2007/059028 WO 20070426
- 主分类号： C12N7/01
- IPC分类号： C12N7/01 ; C12N5/06 ; C12P21/00
摘要：
Known cell lines derived from silkworm exhibit low propagation efficiency of BmNPV. Accordingly, systems using known culture cell lines derived from silkworms take a long time to establish recombinant viruses, and are not suitable for preparation of virus solutions with high titers. The present invention provides a cell line Bme21 (FERM P-20852) that is derived from a silkworm embryo and is highly susceptible to BmNPV or its variant having the same biological characteristics. The present invention also provides a method of producing a recombinant virus, a method of producing a recombinant protein, a method of increasing efficiency of recombinant virus production, and a method of increasing efficiency of recombinant protein production, using the cell line Bme21 or its variant.
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