发明申请
- 专利标题: Method for amplifying genomic DNA
- 专利标题(中): 扩增基因组DNA的方法
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申请号: US12214044申请日: 2008-06-16
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公开(公告)号: US20090311753A1公开(公告)日: 2009-12-17
- 发明人: Takashi Imai , Mayumi Iwakawa , Yuichi Michikawa
- 申请人: Takashi Imai , Mayumi Iwakawa , Yuichi Michikawa
- 申请人地址: JP Chiba-shi
- 专利权人: National Institute of Radiological Sciences
- 当前专利权人: National Institute of Radiological Sciences
- 当前专利权人地址: JP Chiba-shi
- 主分类号: C12P19/34
- IPC分类号: C12P19/34
摘要:
A method for amplifying genomic DNA is provided. The method comprises the steps of: (1) incubating a cell-containing agarose solution at a pH of 9 to 12 and a temperature of 45 to 80° C. to produce a genomic DNA-dispersed agarose solution wherein 0.002 to 1 copies/5 microliter of single-stranded genomic DNA is dispersed; (2) solidifying the genomic DNA-dispersed agarose solution to produce a genomic DNA-dispersed agarose gel and neutralizing a pH of the gel; and (3) adding a DNA polymerase with strand displacement activity, primer and dNTP to the genomic DNA-dispersed agarose gel and incubating the gel at a temperature of 0 to 60° C. to amplify the genomic DNA.
公开/授权文献
- US07678543B2 Method for amplifying genomic DNA 公开/授权日:2010-03-16
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