An optical film has a light incident side and a light emitting side above the light incident side. V shape protrusions disposed side by side are disposed at the light incident side. Collimating units disposed side by side are disposed at the light emitting side. Each of the V shape protrusions and each of the collimating units extend along a predetermined direction. The collimating units are respectively corresponded to the V shape protrusions. Two inclined surfaces of each of the V shape protrusions are respectively a light incident surface and a reflection surface. In each corresponding pair of the V shape protrusion and the collimating unit, a central axis of the collimating unit parallel to the predetermined direction is right above the reflection surface of the V shape protrusion. A backlight module using the optical film is provided to provide a plane light source having high luminance.