- 专利标题: N-Acetylglucosaminyltransferase III Expression in Lower Eukaryotes
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申请号: US12540849申请日: 2009-08-13
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公开(公告)号: US20100016561A1公开(公告)日: 2010-01-21
- 发明人: Piotr Bobrowicz , Stephen R. Hamilton , Tillman U. Gerngross , Stefan Wildt , Byung-Kwon Choi , Juergen Hermann Nett , Robert C. Davidson
- 申请人: Piotr Bobrowicz , Stephen R. Hamilton , Tillman U. Gerngross , Stefan Wildt , Byung-Kwon Choi , Juergen Hermann Nett , Robert C. Davidson
- 申请人地址: US NH Lebanon
- 专利权人: GlycoFi, Inc.
- 当前专利权人: GlycoFi, Inc.
- 当前专利权人地址: US NH Lebanon
- 主分类号: C07K14/00
- IPC分类号: C07K14/00 ; C12P21/06
摘要:
The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells exhibit GnTIII activity, which produce bisected N-glycan structures and may be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.