发明申请
US20100021899A1 METHOD OF ASSAYING TARGET SUBSTANCE IN SAMPLE, APTAMER MOLECULE AND METHOD OF CONSTRUCTING THE SAME
失效
测定样品中目标物质的方法，APTAMER分子及其构建方法
- 专利标题： METHOD OF ASSAYING TARGET SUBSTANCE IN SAMPLE, APTAMER MOLECULE AND METHOD OF CONSTRUCTING THE SAME
- 专利标题（中）： 测定样品中目标物质的方法，APTAMER分子及其构建方法
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申请号： US12443053申请日： 2007-09-27
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公开(公告)号： US20100021899A1公开(公告)日： 2010-01-28
- 发明人: Kazunori Ikebukuro , Koji Sode
- 申请人： Kazunori Ikebukuro , Koji Sode
- 申请人地址： JP FUCHU-SHI TOKYO
- 专利权人： NATIONAL UNIVERSITY CORPORATION TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY
- 当前专利权人： NATIONAL UNIVERSITY CORPORATION TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY
- 当前专利权人地址： JP FUCHU-SHI TOKYO
- 国际申请： PCT/JP2007/068767 WO 20070927
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12N15/115
摘要：
A method for measuring a test substance whereby the test substance in a sample can be specifically measured without using an antibody against the test substance; an aptamer molecule used therefor; and a method for creating the aptamer are disclosed. An aptamer capable of hybridizing with an oligonucleotide when it is bound to a test substance, but is incapable of hybridizing with the oligonucleotide when it is not bound to the test substance, is utilized. The aptamer is brought into contact with a sample, and the aptamer bound to the test substance is brought into contact with an immobilized oligonucleotide which hybridizes with the aptamer, to bind the aptamer to a solid phase, followed by measurement of the aptamer immobilized on the solid phase.
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