The present invention relates to an extract of Stewartia koreana and use thereof. More particularly, it relates to an extract of Stewartia koreana which is extracted with any one selected from the group consisting of water, a C1-4 low alcohol, a polar solvent, a non-polar solvent and a mixture thereof, and a pharmaceutical composition for promoting angiogenesis or tissue regeneration and a cosmetic for improving wrinkles comprising the same as an effective ingredient. The extract of Stewartia koreana according to the present invention promotes migration and multiplication of endothelial cell and shows excellent effect in angiogenesis and wound healing and thus, is useful in treatment or prevention of diseases which requires angiogenesis for healing of wounded and frostbitten region, wound healing after surgical operation, and treatment and prevention of gastric ulcer, ischaemic heart diseases and hair loss. Also, the extract shows the dermal tissue regenerating effect and thus, is useful as a cosmetic for improving wrinkles.