发明申请
US20100159545A1 METHOD FOR PRODUCTION OF COROSOLIC ACID IN SUSPENSION CULTURE OF PLANT CELLS
有权
用于在植物细胞悬浮培养物中生产赤霉酸的方法
- 专利标题: METHOD FOR PRODUCTION OF COROSOLIC ACID IN SUSPENSION CULTURE OF PLANT CELLS
- 专利标题(中): 用于在植物细胞悬浮培养物中生产赤霉酸的方法
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申请号: US11993316申请日: 2006-06-30
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公开(公告)号: US20100159545A1公开(公告)日: 2010-06-24
- 发明人: Chang-Heon Kim , Jin-An Kim , Jai-Young Song , Ho-Joon Choi
- 申请人: Chang-Heon Kim , Jin-An Kim , Jai-Young Song , Ho-Joon Choi
- 申请人地址: KR Seoul
- 专利权人: SAMYANG GENEX CORPORATION
- 当前专利权人: SAMYANG GENEX CORPORATION
- 当前专利权人地址: KR Seoul
- 优先权: KR10-2005-0058377 20050630
- 国际申请: PCT/KR06/02574 WO 20060630
- 主分类号: C12P7/42
- IPC分类号: C12P7/42 ; C12N5/04
摘要:
The present invention relates to a method of producing corosolic acid by using plant cells that produce corosolic acid. More particularly, the present invention relates to a method of producing corosolic acid by using plant cell suspension cultures comprising the steps of: inducing a callus from a tissue of a plant producing corosolic acid; preparing a cell line capable of being cultured in liquid culture medium from the induced callus; culturing the cell line in a suspension culture; and isolating corosolic acid from the culture solution. The present invention has advantage of maximizing productivity by utilizing two-stage culture, treatment with inducing agent, and high cell-density culture in the suspension culture of plant cells producing corosolic acid.