发明申请
- 专利标题: URINE PRETREATMENT AGENT FOR URINARY PROTEIN QUANTITATION, URINE PRETREATMENT METHOD, AND URINARY PROTEIN QUANTITATION METHOD
- 专利标题(中): 尿素预处理剂尿素蛋白质定量,尿液预处理方法和尿素蛋白质定量方法
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申请号: US12680479申请日: 2008-09-26
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公开(公告)号: US20100233738A1公开(公告)日: 2010-09-16
- 发明人: Masanori Hara , Akihiko Saito , Shinya Ogasawara , Yoshiaki Hirayama , Hiroyuki Kurosawa
- 申请人: Masanori Hara , Akihiko Saito , Shinya Ogasawara , Yoshiaki Hirayama , Hiroyuki Kurosawa
- 申请人地址: JP Niigataq-shi, Nigata JP Niigata-shi, Niigata JP Chuo-ku, Tokyo
- 专利权人: Niigata University,Masanori HARA,Denka Seiken Co., Ltd.
- 当前专利权人: Niigata University,Masanori HARA,Denka Seiken Co., Ltd.
- 当前专利权人地址: JP Niigataq-shi, Nigata JP Niigata-shi, Niigata JP Chuo-ku, Tokyo
- 优先权: JP2007-251707 20070927
- 国际申请: PCT/JP2008/067422 WO 20080926
- 主分类号: G01N33/53
- IPC分类号: G01N33/53 ; G01N33/00
摘要:
The present invention is intended to develop a urine pretreatment agent, a urine pretreatment method, and a urinary protein quantitation method which reduce or cancel the influences of urine pH variations, cancel the influences of precipitates of urinary inorganic salts, and solubilize membrane proteins. The present invention provides: a urine pretreatment agent for urinary protein quantitation, comprising a buffer, a chelating agent, and a surfactant; a urine pretreatment method comprising a step of mixing 10 to 1000 parts by mass of the urine pretreatment agent of the present invention with 100 parts by mass of urine; and a urinary protein quantitation method comprising steps of: mixing 10 to 1000 parts by mass of the urine pretreatment agent of the present invention with 100 parts by mass of urine; and then measuring the protein concentration.
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