A method (50) for quantitative 3D contrast enhanced ultrasound (CEUS) analysis includes acquiring (54) an initial pair of ultrasound contrast and tissue images of an anatomy. A region of interest (ROI) or volume of interest (VOI) is established (56) in the initial acquired tissue image, which becomes the baseline tissue image. The established ROI/VOI is automatically registered (58) from the initial tissue image to the initial contrast image, which becomes a baseline contrast image. Quantitative analysis is performed (60) on the ROI/VOI of the baseline contrast image. The method further includes acquiring (62) a next ultrasound contrast and tissue image pair, corresponding to an i th current contrast and tissue image pair. Frame-to-frame registration is established (64) between (i) the current tissue image and (ii) the baseline tissue image, the frame-to-frame registration being used (66) between the current tissue image and the baseline tissue image to transfer the ROI/VOI from (i) the baseline contrast image to (ii) the current contrast image, thereby creating a transformed ROI/VOI in the current contrast image. Quantitative analysis is then performed (68) on the transformed ROI/VOI of the current contrast image.