The present invention discloses a method using a microfluidic chip to sort high motility sperm. In the present invention, sperm and a medium are respectively injected into a microchannel of a microfluidic chip via several inlets. Owing to the characteristic of microfluidics, the sperm and the medium form a sperm laminar flow and medium laminar flow in the microchannels; the sperm laminar flow and the medium laminar flow are parallel to each other. The higher motile sperm may pass through at least one laminar flow within a limited time, whereby different motility levels of sperm can be respectively collected from different outlets.