发明申请
US20100323906A1 NANOPLASMONIC MOLECULAR RULER FOR NUCLEASE ACTIVITY AND DNA FOOTPRINTING
有权
用于核酸活性的NANOPLASMONIC分子规则和DNA加工
- 专利标题: NANOPLASMONIC MOLECULAR RULER FOR NUCLEASE ACTIVITY AND DNA FOOTPRINTING
- 专利标题(中): 用于核酸活性的NANOPLASMONIC分子规则和DNA加工
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申请号: US12440579申请日: 2007-09-14
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公开(公告)号: US20100323906A1公开(公告)日: 2010-12-23
- 发明人: Fanqing Frank Chen , Gang L. Liu , Luke P. Lee
- 申请人: Fanqing Frank Chen , Gang L. Liu , Luke P. Lee
- 国际申请: PCT/US07/20026 WO 20070914
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C07H21/02 ; C40B40/08 ; C40B30/04 ; C07H21/04
摘要:
This invention provides a nanoplasmonic molecular ruler, which can perform label-free and real-time monitoring of nucleic acid (e.g., DNA) length changes and perform nucleic acid footprinting. In various embodiments the ruler comprises a nucleic acid attached to a nanoparticle, such that changes in the nucleic acid length are detectable using surface plasmon resonance. The nanoplamonic ruler provides a fast and convenient platform for mapping nucleic acid -protein interactions, for nuclease activity monitoring, and for other footprinting related methods.