An optical fiber holder assembly for holding an optical fiber having any one of several different fiber constructions comprises a base and a fiber clamping mechanism. The clamping mechanism includes a clamping portion configured to clamp the optical fiber, the clamping portion including a first clamping plate hingedly coupled to a portion of the base. A first fiber entrance guide is formed in an entrance end of the fiber clamping mechanism to receive and guide the optical fiber to a first fiber channel formed in the base. A second fiber entrance guide is formed in the entrance end of the fiber clamping mechanism to receive and guide the optical fiber to a second fiber channel formed in the base. The first clamping portion includes rust and second compliant gripping pads, the first gripping pad disposed on the base and the second gripping pad disposed in the first clamping plate such that the gripping pads overlap each other when the first clamping plate is placed in a closed position.