发明申请
US20110065138A1 Quantitative Method For Detecting Yessotoxins In Fishery Products On Based On The Activation That The Toxin Produces In Cellular Phosphodiesterases And Therapeutic Use Of This Activation
审中-公开
基于毒素在细胞磷酸二酯酶中产生的激活和该激活的治疗用途来检测渔业产品中的Yessotoxins的定量方法
- 专利标题： Quantitative Method For Detecting Yessotoxins In Fishery Products On Based On The Activation That The Toxin Produces In Cellular Phosphodiesterases And Therapeutic Use Of This Activation
- 专利标题（中）： 基于毒素在细胞磷酸二酯酶中产生的激活和该激活的治疗用途来检测渔业产品中的Yessotoxins的定量方法
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申请号： US10566050申请日： 2004-07-21
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公开(公告)号： US20110065138A1公开(公告)日： 2011-03-17
- 发明人: Luis Miguel Botana López , Amparo Alfonso Rancaño , Maria Jose Pazos Guldris , Mercedes Rodrugues Vieytes , Maria Isabel Loza Garcia , JUan Manuel Vieites Baptista De Sousa
- 申请人： Luis Miguel Botana López , Amparo Alfonso Rancaño , Maria Jose Pazos Guldris , Mercedes Rodrugues Vieytes , Maria Isabel Loza Garcia , JUan Manuel Vieites Baptista De Sousa
- 申请人地址： ES Santiago de Compostela (La Coruna)
- 专利权人： UNIVERSIDADE DE SANTIAGO DE COMPOSTELA
- 当前专利权人： UNIVERSIDADE DE SANTIAGO DE COMPOSTELA
- 当前专利权人地址： ES Santiago de Compostela (La Coruna)
- 优先权： ESP200301773 20030725
- 国际申请： PCT/ES2004/000343 WO 20040721
- 主分类号： C12Q1/42
- IPC分类号： C12Q1/42 ; A61K31/47
摘要：
The present invention relates to a quantitative method for detecting yessotoxins in fishery products based on the activation the toxin produces on cellular phosphodiesterases and the therapeutic use of this activation. The cellular target of yessotoxin (YTX) and its analogs is the activation of phosphodiesterases (PDEs). The PDEs-YTX bond produces a measurable signal. The bond can be quantified by means of an affinity biosensor or by fluorescence. The biosensor detects biomolecular interactions and allows determining the presence of YTX due to its interaction with PDEs. The variations in the degradation rate of the fluorescent derivative anthraniloyl-cAMP are determined by means of plate fluorescence. The rate at which the PDEs degrade this molecule increases in the presence of YTX. YTX inhibits immunological activation of mastocytes in rats and induces a cytotoxic effect in human hepatocarcinoma cells, which implies two therapeutic uses of YTXs as an antiallergic and antitumor compound.
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