发明申请
- 专利标题： Expression Vector
- 专利标题（中）： 表达载体
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申请号： US12995924申请日： 2009-06-01
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公开(公告)号： US20110086391A1公开(公告)日： 2011-04-14
- 发明人: Bhupendra Vallabh Kara , Christopher David John Lennon
- 申请人： Bhupendra Vallabh Kara , Christopher David John Lennon
- 优先权： GB0810154.5 20080604
- 国际申请： PCT/GB2009/001372 WO 20090601
- 主分类号： C12P21/00
- IPC分类号： C12P21/00 ; C12N15/63 ; C12N1/21 ; C07H21/04 ; C07K14/00
摘要：
An expression vector for expressing a target polypeptide in a prokaryotic cell is provided. The vector comprises a promoter operably linked to a polynucleotide encoding the target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of: a) MLKRSSWLATLGLLTVASVSTIVYA; b) MKKATFITCLLAVLLVSNPIWNA; c) MKVSAAALAVILIATALCAPASA; d) MKVSTAFLCLLLTVSAFSAQVLA; and e) MKCLLLALGLALACAAQA. Processes for expressing polypeptides and prokaryotic microorganisms comprising such vectors are also provided.