发明申请
US20110092680A1 METHOD OF SEPARATING AND PURIFYING CELLULAR COMPONENTS USING NON-COVALENT BOND BETWEEN CUCURBITAL DERIVATIVE AND GUEST COMPOUND AND APPARATUS USING THE SAME
有权
使用CUCURBITAL DERIVATIVE和顾客化合物之间的非共价键和使用其的装置分离和纯化细胞组分的方法
- 专利标题: METHOD OF SEPARATING AND PURIFYING CELLULAR COMPONENTS USING NON-COVALENT BOND BETWEEN CUCURBITAL DERIVATIVE AND GUEST COMPOUND AND APPARATUS USING THE SAME
- 专利标题(中): 使用CUCURBITAL DERIVATIVE和顾客化合物之间的非共价键和使用其的装置分离和纯化细胞组分的方法
-
申请号: US12988254申请日: 2009-04-15
-
公开(公告)号: US20110092680A1公开(公告)日: 2011-04-21
- 发明人: Ki Moon Kim , Don Wook Lee , Sung Ho Ryu , Sang Hoon Ha , Hyun Tae Jung , Banerjee Mainak , Narayanan Selvapalam , Kyeng Min Park
- 申请人: Ki Moon Kim , Don Wook Lee , Sung Ho Ryu , Sang Hoon Ha , Hyun Tae Jung , Banerjee Mainak , Narayanan Selvapalam , Kyeng Min Park
- 申请人地址: KR Pohang-si, Kyungsangbuk-do
- 专利权人: POSTECH ACADEMY-INDUSTRY FOUNDATION
- 当前专利权人: POSTECH ACADEMY-INDUSTRY FOUNDATION
- 当前专利权人地址: KR Pohang-si, Kyungsangbuk-do
- 优先权: KR10-2008-0035223 20080416
- 国际申请: PCT/KR2009/001947 WO 20090415
- 主分类号: C07K1/30
- IPC分类号: C07K1/30 ; C12N9/00 ; C12N7/00 ; B01J8/00
摘要:
Provided is a method of separating cellular components, the method including: a) contacting a guest compound-bound reactive compound with cells; b) lysing the cells; c) adding a host compound-bound solid phase to a solution including the lysates of the cells to prepare a mixture; d) separating binding pairs of the guest compound bound to cellular components and the host compound bound to the solid phase from the mixture, and purifying the binding pairs; and e) separating the cellular components from the binding pairs, wherein the guest compound-bound reactive compound is obtained through a covalent bond between a reactive compound and a guest compound represented by Formula 2 below guest, the host compound-bound solid phase is obtained through a covalent bond between a solid phase and a host compound represented by Formula 1 below, and the reactive compound includes at least one selected from the group consisting of a biomolecule, N-hydroxysuccimide, an antigen, an antibody, an aptamer, folic acid, transferrin, and any mixtures thereof.
公开/授权文献
信息查询