发明申请
- 专利标题： GENE SITE SATURATION MUTAGENESIS
- 专利标题（中）： 基因座饱和度
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申请号： US12817616申请日： 2010-06-17
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公开(公告)号： US20110165627A1公开(公告)日： 2011-07-07
- 发明人: Jay M. SHORT
- 申请人： Jay M. SHORT
- 申请人地址： US CA San Diego
- 专利权人： Verenium Corporation
- 当前专利权人： Verenium Corporation
- 当前专利权人地址： US CA San Diego
- 主分类号： C12P19/34
- IPC分类号： C12P19/34
摘要：
A method for producing progeny polynucleotides and polypeptides by Gene Site Saturation Mutagenesis (GSSM). The method provides a set of degenerate primers corresponding to codons of a template polynucleotide, and performs polymerase elongation to produce progeny polynucleotides, which contain sequences corresponding to the degenerate primers. The progeny polynucleotides can be expressed and screened for directed evolution.
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