发明申请
- 专利标题： SINGLE-STRANDED AND DOUBLE-STRANDED OLIGONUCLEOTIDES COMPRISING A METAL-CHELATING LIGAND
- 专利标题（中）： 包含金属链条的单条和双拉链的双绞线
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申请号： US12671677申请日： 2008-07-30
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公开(公告)号： US20110250138A1公开(公告)日： 2011-10-13
- 发明人: Yupeng Fan , Martin Maier , Rajendra K. Pandey , Muthiah Manoharan , Kallanthottathil G. Rajeev
- 申请人： Yupeng Fan , Martin Maier , Rajendra K. Pandey , Muthiah Manoharan , Kallanthottathil G. Rajeev
- 申请人地址： US MA Cambridge
- 专利权人： ALNYLAM PHARMACEUTICALS, INC.
- 当前专利权人： ALNYLAM PHARMACEUTICALS, INC.
- 当前专利权人地址： US MA Cambridge
- 国际申请： PCT/US2008/071576 WO 20080730
- 主分类号： A61K49/00
- IPC分类号： A61K49/00 ; C07F5/00
摘要：
The invention provides modified oligonucleotides of formula (I), comprising at least one metal chelator which provides a powerful tool for study of the pharmacokinetics of siRNA and its correlation with in vivo activity. The chelated metals provide luminescent properties enable detection of the oligonucleotides through the use of time-resolved fluorescent quenching based on energy transfer from the metal ion to a nonfluorescent quencher which can be used as non-isotopic labels of oligonucleotides for diagnostics and evaluation of cellular uptake.