The present invention discloses an optical fiber connection device, comprising: a base plate; at least one adapter for receiving optical fiber connectors; a rotating and fixing mechanism being provided on the base plate and being connected with the adapters, the rotating and fixing mechanism comprising: a fixing portion for fixedly connecting with the adapter; and a rotating portion being rotatably connected to the base plate, through which the mechanism can rotate between an operation position and a receiving/releasing position; and a positioning member for stably holding the rotating and fixing mechanism in the operation position or the receiving/releasing position, and limiting the rotation angle of the mechanism and that of the adapter within a predetermined angle range.