A cartridge for measuring a biological sample component and an apparatus for measuring a biological sample component are provided. The cartridge for measuring a biological sample component includes: an upper cartridge having a first chamber storing a biological sample, a first channel connected to the first chamber and delivering air pressure to the first chamber, and a third channel transferring the biological sample; and a lower cartridge having a third chamber storing a first reagent, a fifth chamber storing a second reagent, a fifth channel connecting the third and fifth chambers and transferring the second reagent to the third chamber, an eleventh channel delivering the biological sample delivered through the third channel to the third chamber, and a thirteenth channel delivering air pressure to the fifth chamber, wherein when the upper and lower cartridges are bounded by an external force, the third channel and the eleventh channel are connected.