[Object] An object of the invention is to provide a multilayer stretched film with barrier property that is free from deterioration of barrier properties attributable to dimensional change caused by moisture absorption, and bending, etc.[Method for Achieving the Object] A multilayer stretched film with barrier property obtained by biaxially stretching a multilayer laminate comprising at least two layers, namely, a polyester-based resin layer (Layer A) and a polyamide-based resin layer (Layer B) to obtain a biaxially stretched multilayer film; and providing a deposition layer (Layer C) on at least one surface of the biaxially stretched multilayer film; Layer A containing a crystalline polyester; Layer B containing 70 to 99 wt % of aliphatic polyamide and 1 to 30 wt % of aromatic polyamide; and Layer C containing an inorganic substance. [Selected Drawings] None