Provided are a computer program product, method, and system for capturing information on a rendered user interface including user activatable content. A user interface is executed on the computer system. An initial representation is generated of a rendered user interface. A determination is made of locations rendered in the user interface associated with user activatable content, wherein user activatable content is rendered in response to user selection of a location in the user interface associated with the user activatable content. For each determined location, content is captured from the activatable content rendered in response to the user selecting the determined location to create a captured content object having the captured content. The captured content objects and the initial representation are stored. The initial representation and the captured content objects are processed to generate output to render the initial representation of the user interface and the captured content from the captured content objects.