Provided is an analysis method in which increase in a background can be prevented in a simple manner without cost. The analysis method of the present invention is carried out using a sample analysis tool 10 including a development member 11 in which a developing solution supply portion 12, a sample supply portion 13, and a detection portion 14 in which a substance 17 that specifically binds to an analyte 16 in a sample is immobilized are provided in this order from upstream to downstream along the flow of a developing solution. A sample solution is supplied to the sample supply portion 13, and a developing solution is supplied to the developing solution supply portion 12 simultaneously with the supply of the sample solution or prior to the supply of the sample solution. By the development of the developing solution in the development member 11 in the presence of a labeled specifically binding substance 15, the sample solution is introduced to the detection portion 14. In the detection portion 14, a complex 18 of the immobilized specifically binding substance 17, the analyte 16, and the labeled specifically binding substance 15 is formed. The analyte 16 is analyzed by detecting a label 19 in the complex 18.