An analysis chip includes a carrier having a surface on which a selective binding substance(s) is(are) immobilized; a vessel holding a solution containing a test substance(s) which react(s) with the selective binding substance(s); and particles for stirring the solution, the particles being sealed within a space formed between the carrier and the vessel wherein a separator which allows passage therethrough of the solution containing the test substance(s), but does not allow passage therethrough of the particles is arranged in the space to separate the surface of the carrier on which the selective binding substance(s) is(are) immobilized and the particles.