发明申请
- 专利标题: METHOD FOR CULTIVATION OF GENETICALLY-MODIFIED PLANT
- 专利标题(中): 基因改造植物的培育方法
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申请号: US13147436申请日: 2009-08-26
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公开(公告)号: US20120054923A1公开(公告)日: 2012-03-01
- 发明人: Saori Kasahara , Masafumi Wasai , Koichi Sugita , Teruhisa Shimada
- 申请人: Saori Kasahara , Masafumi Wasai , Koichi Sugita , Teruhisa Shimada
- 申请人地址: JP Tokyo
- 专利权人: NIPPON PAPER INDUSTRIES CO., LTD
- 当前专利权人: NIPPON PAPER INDUSTRIES CO., LTD
- 当前专利权人地址: JP Tokyo
- 优先权: JP2009-021846 20090202
- 国际申请: PCT/JP2009/064876 WO 20090826
- 主分类号: A01H5/10
- IPC分类号: A01H5/10 ; A01G31/00 ; A01H5/00
摘要:
The present invention provides a method for cultivation of a genetically-modified plant that can highly produce a desired protein. More specifically, the present invention provides a method for cultivation of a genetically-modified plant comprising: cultivating the genetically-modified plant in a medium, wherein the genetically-modified plant is transformed by introducing an expression vector comprising a promoter regulating expression of RNA and a seed storage protein isolated from a plant that highly expresses RNA and the seed storage protein under a high nitrogen condition; and a polynucleotide encoding an objective protein, and wherein the medium is adjusted so that nitrate nitrogen is 70 mg/L to 750 mg/L and/or ammonium nitrogen is 70 mg/L to 750 mg/L for a definite period in a period from 30 days before an expected flowering date to a date on or before flowering of the genetically-modified plant.
公开/授权文献
- US09353379B2 Method for cultivation of genetically-modified plant 公开/授权日:2016-05-31
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