发明申请
- 专利标题： ENZYME PREPARATION CONTAINING THERMOSTABLE DNA POLYMERASE, METHOD FOR PRODUCING SAME, AND METHOD FOR DETECTING SUBJECT ORGANISM TO BE DETECTED
- 专利标题（中）： 含有热稳定性DNA聚合酶的酶制剂，其生产方法和检测待检测的有机体的方法
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申请号： US13144175申请日： 2009-01-15
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公开(公告)号： US20120094296A1公开(公告)日： 2012-04-19
- 发明人: Homare Tabata , Hiroshi Minami , Hideki Niimi , Isao Kitajima , Tomohiro Ueno , Shiroh Hayashi , Masashi Mori
- 申请人： Homare Tabata , Hiroshi Minami , Hideki Niimi , Isao Kitajima , Tomohiro Ueno , Shiroh Hayashi , Masashi Mori
- 申请人地址： JP Toyama-shi, Toyama JP Sunagawa-shi, Hikkaido
- 专利权人： NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYA MA,HOKKAIDO MITSUI CHEMICALS INC.
- 当前专利权人： NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYA MA,HOKKAIDO MITSUI CHEMICALS INC.
- 当前专利权人地址： JP Toyama-shi, Toyama JP Sunagawa-shi, Hikkaido
- 优先权： JP2009-006556 20090115; JP2009-023707 20090204; JP2009-040052 20090223; JP200--181755 20090804
- 国际申请： PCT/JP2010/050443 WO 20090115
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12N9/12
摘要：
Disclosed is a thermostable DNA polymerase preparation which can illimitably reduce the risk of false positivity in the detection of a subject microorganism utilizing a gene amplification reaction and therefore enables the selective amplification of DNA for detecting the subject microorganism even when the amount of the subject microorganism is small and therefore the amount of DNA collected therefrom is extremely small, and can be produced at a reduced cost. Also disclosed is a method for quantifying or quantifying/identifying a subject organism to be detected rapidly, conveniently and with high sensitivity using the preparation of the present invention.