发明申请
US20120094343A1 Gene Knockout Mesophilic and Thermophilic Organisms, and Methods of Use Thereof
审中-公开
基因敲除嗜中性和嗜热生物及其使用方法
- 专利标题: Gene Knockout Mesophilic and Thermophilic Organisms, and Methods of Use Thereof
- 专利标题(中): 基因敲除嗜中性和嗜热生物及其使用方法
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申请号: US13129085申请日: 2009-11-12
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公开(公告)号: US20120094343A1公开(公告)日: 2012-04-19
- 发明人: David A. Hogsett , Vineet Rajgarhia , Arthur J. Shaw, IV , Nicky C. Caiazza
- 申请人: David A. Hogsett , Vineet Rajgarhia , Arthur J. Shaw, IV , Nicky C. Caiazza
- 申请人地址: US NH Lebanon
- 专利权人: Mascoma Corporation
- 当前专利权人: Mascoma Corporation
- 当前专利权人地址: US NH Lebanon
- 国际申请: PCT/US09/64128 WO 20091112
- 主分类号: C12P7/56
- IPC分类号: C12P7/56 ; C12P7/54 ; C12N1/21 ; C12N15/11 ; C12N15/74
摘要:
One aspect of the invention relates to a genetically modified thermophilic or mesophilic microorganism, wherein a first native gene is partially, substantially, or completely deleted, silenced, inactivated, or down-regulated, which first native gene encodes a first native enzyme involved in the metabolic production of an organic acid or a salt thereof, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce lactate or acetate as a fermentation product. In certain embodiments, the aforementioned microorganism further comprises a first non-native gene, which first non-native gene encodes a first non-native enzyme involved in the metabolic production of lactate or acetate. Another aspect of the invention relates to a process for converting lignocellulosic biomass to lactate or acetate, comprising contacting lignocellulosic biomass with a genetically modified thermophilic or mesophilic microorganism.