发明申请
- 专利标题： CHO/CERT CELL LINES
- 专利标题（中）： CHO / CERT细胞系
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申请号： US13318509申请日： 2010-05-04
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公开(公告)号： US20120100553A1公开(公告)日： 2012-04-26
- 发明人: Lore Florin , Eric Becker , Hitto Kaufmann
- 申请人： Lore Florin , Eric Becker , Hitto Kaufmann
- 申请人地址： DE Ingelheim am Rhein
- 专利权人： BOEHRINGER INGELHEIM INTERNATIONAL GMBH
- 当前专利权人： BOEHRINGER INGELHEIM INTERNATIONAL GMBH
- 当前专利权人地址： DE Ingelheim am Rhein
- 优先权： EP09159439.0 20090505; EP09161907.2 20090604
- 国际申请： PCT/EP10/56009 WO 20100504
- 主分类号： C12N5/10
- IPC分类号： C12N5/10 ; C12P21/00 ; C12Q1/68
摘要：
The invention concerns the field of cell culture technology. The invention describes production host cell lines comprising vector constructs comprising a CERT S132 A expression cassette. Those cell lines have improved growth characteristics and high CERT S132A expression levels. The invention especially concerns two cell lines deposited with the DSMZ under the number DSM ACC2989 (CHO/CERT 2.20) and DSM AC-C2990 (CHO/CERT 2.41). The invention further concerns a method of generating such preferred production host cells and a method of producing proteins using the two cell lines deposited with the DSMZ under the number DSM ACC2989 (CHO/CERT 2.20) and DSM ACC2990 (CHO/CERT 2.41).
公开/授权文献
- US09340592B2 CHO/CERT cell lines 公开/授权日：2016-05-17
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