An ambient-temperature self-curable fluorine containing aqueous-based polyurethane dispersion, a method of manufacturing the same and its modified coated film applications are provided. The ambient-temperature self-curable fluorine containing aqueous-based PU dispersion includes water, an ambient temperature cross-linking agent and a fluorine containing PU resin dispersed in water phase. The fluorine containing PU resin includes A includes octa-fluoropentanol, hexafluoroisopropanol, trifluoroethanol, tetrafluoropropanol or trifluoroethylamine.Treated fabric with the self-cured fluorine containing aqueous-based PU resin and it becomes a long-lasting water-repellent and stain-proof.