发明申请
- 专利标题: Method for Generating Aptamers with Improved Off-Rates
- 专利标题(中): 用改进的脱离率产生适配器的方法
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申请号: US13382493申请日: 2010-07-09
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公开(公告)号: US20120115752A1公开(公告)日: 2012-05-10
- 发明人: Dominic Zichi , Sheri K. Wilcox , Chris Bock , Daniel J. Schneider , Bruce Eaton , Larry Gold , Thale C. Jarvis , Jeffrey D. Carter
- 申请人: Dominic Zichi , Sheri K. Wilcox , Chris Bock , Daniel J. Schneider , Bruce Eaton , Larry Gold , Thale C. Jarvis , Jeffrey D. Carter
- 申请人地址: US CO Boulder
- 专利权人: SOMALOGIC, INC.
- 当前专利权人: SOMALOGIC, INC.
- 当前专利权人地址: US CO Boulder
- 国际申请: PCT/US10/41540 WO 20100709
- 主分类号: C40B30/04
- IPC分类号: C40B30/04 ; C12Q1/68 ; C12Q1/70 ; C07H21/00 ; C12Q1/02 ; B82Y15/00
摘要:
The present disclosure describes the identification and use of aptamers and photoaptamers having slower dissociation rate constants than those obtained using previously described methods. Specifically, the present disclosure describes methods for the identification and use of aptamers to one or more targets within a histological or cytological sample, which have slow rates of dissociation. The aptamers may be used to assess localization, relative density, and presence or absence of one or more targets in cytological and histological samples. Targets may be selected that are specific and diagnostic of a given disease state for which the sample was collected. The aptamers may also be used to introduce target specific signal moieties. In addition to target identification, the aptamers may be used to amplify signal generation through a variety of methods.
公开/授权文献
- US08975026B2 Method for generating aptamers with improved off-rates 公开/授权日:2015-03-10