A system and method for hyperspectral imaging to detect hazardous agents including explosive agents. A system comprising a tunable laser, a collection optics, and one or more hyperspectral imaging detectors configured for hyperspectral imaging of a target comprising an unknown material. A method comprising illuminating a target comprising an unknown material via a tunable laser to thereby generate a plurality of interacted photons. Detecting said interacted photons to generate at least one hyperspectral image representative of the target. One or more hyperspectral images may be obtained including SWIR, MWIR, and LWIR hyperspectral images. Algorithms and chemometric techniques may be applied to assess the hyperspectral images to identify the unknown material as comprising an explosive agent or a non-explosive agent. A video imaging device may also be configured to provide a video image of an area of interest, which may be assessed to identify a target for interrogation using hyperspectral imaging.