A three-dimensional video system includes a panel driving module, a signal transmitter and a shutter glasses. The panel driving module includes a timing controller, and a control unit, for generating a control signal. The signal transmitter is utilized for transmitting a radio frequency control signal according to the control signal. The shutter glasses includes a receiver, a calibrating and selecting unit, for alternating the receiver between a first operating status and a second operating status, and generating a calibration signal according to the received radio frequency control signal, and an LCD glass, for operating according to the calibration signal.