A compact imaging optical system having a light-weight focusing lens is provided. The imaging optical system includes, in order from an object side to an image side, a front unit in which a negative lens element is arranged closest to the object side, an aperture diaphragm, and a rear unit having positive optical power. In focusing from an infinite distance object to a short distance object, the negative lens element arranged closest to the object side and the aperture diaphragm are fixed with respect to an image surface, and a part of lens elements included in the front unit or the rear unit moves along an optical axis. The imaging optical system satisfies the following condition (1): 0.9