发明申请
US20120288913A1 NOVEL FUSION PARTNERS FOR THE PURPOSE OF CRYSTALLIZING G-PROTEIN COUPLED RECEPTORS
审中-公开
用于结晶G蛋白偶联受体的目的的新型融合合作伙伴
- 专利标题： NOVEL FUSION PARTNERS FOR THE PURPOSE OF CRYSTALLIZING G-PROTEIN COUPLED RECEPTORS
- 专利标题（中）： 用于结晶G蛋白偶联受体的目的的新型融合合作伙伴
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申请号： US13470104申请日： 2012-05-11
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公开(公告)号： US20120288913A1公开(公告)日： 2012-11-15
- 发明人: Michael A. HANSON , Christopher B. Roth , Raymond C. Stevens , Joshua M. Kunken , Mark T. Griffith , Aaron A. Thompson , Wei Liu , Fei Xu , Vsevolod Katritch
- 申请人： Michael A. HANSON , Christopher B. Roth , Raymond C. Stevens , Joshua M. Kunken , Mark T. Griffith , Aaron A. Thompson , Wei Liu , Fei Xu , Vsevolod Katritch
- 申请人地址： US CA La Jolla US CA San Diego
- 专利权人： The Scripps Research Institute,RECEPTOS, INC.
- 当前专利权人： The Scripps Research Institute,RECEPTOS, INC.
- 当前专利权人地址： US CA La Jolla US CA San Diego
- 主分类号： C12N9/96
- IPC分类号： C12N9/96 ; G06F17/30 ; C07K19/00
摘要：
GPCR-fusion partner proteins comprising G protein coupled receptors (GPCRs) of GPCRs and fusion partners such as rubredoxin, cytochrome b562 RIL (Bril, bRIL, BRIL), T4 lysozyme C-terminal fragment (Cterm-T4L), flavodoxin, or xylanase either substituted for some or all of the third intracellular loop of the GPCR between the fifth and sixth helix of the GPCR are described or attached to an terminus or C terminus of the GPCR. GPCR-fusion partner proteins in crystalline form, optionally of a quality suitable for x-ray crystallographic structure determination of the GPCR, are described. Methods of using fusion partners in GPCR-fusion partner proteins to support crystallization of GPCR-fusion partner proteins for x-ray crystallographic structure determination of the GPCR, are described. Methods of identifying other suitable fusion partners through screening of protein data banks are also described.
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