A time synchronization method and a time synchronization device in a passive optical network (PON), and a PON are provided. The method includes receiving a synchronization packet sent after time synchronization of an optical line terminal (OLT) with a master clock (MC) is achieved, wherein the synchronization packet carries a timestamp TMt1i determined after the time synchronization of the OLT is achieved, adjusting a local clock according to the timestamp to achieve time synchronization of an optical network unit/optical network terminal (ONU/ONT) with the OLT, and after the time synchronization of the OLT is achieved, instructing an slave clock (SC) to perform time synchronization. A time synchronization device and a time synchronization system for implementing the method in a PON are further provided.