发明申请
- 专利标题: METHODS FOR LOCALIZED IN SITU DETECTION OF mRNA
- 专利标题(中): 用于局部检测mRNA的方法
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申请号: US13542378申请日: 2012-07-05
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公开(公告)号: US20130004953A1公开(公告)日: 2013-01-03
- 发明人: Mats Nilsson Bernitz , Chatarina Larsson , Ida Gundberg
- 申请人: Mats Nilsson Bernitz , Chatarina Larsson , Ida Gundberg
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; G01N21/64
摘要:
The present invention relates to the detection of RNA in a sample of cells. More particularly, the present invention relates to the localized detection of RNA in situ. The method relies on the conversion of RNA to complementary DNA prior to the targeting of the cDNA with a padlock probe(s). The hybridization of the padlock probe(s) relies on the nucleotide sequence of the cDNA which is derived from the corresponding nucleotide sequence of the target RNA. Rolling circle amplification of the subsequently circularized padlock probe produces a rolling circle product which may be detected. Advantageously, this allows the RNA to be detected in situ.
公开/授权文献
- US08551710B2 Methods for localized in situ detection of mRNA 公开/授权日:2013-10-08