发明申请
US20130045492A1 Methods For Making Fully Human Bispecific Antibodies Using A Common Light Chain
审中-公开
使用普通轻链制备全人类双特异性抗体的方法
- 专利标题: Methods For Making Fully Human Bispecific Antibodies Using A Common Light Chain
- 专利标题(中): 使用普通轻链制备全人类双特异性抗体的方法
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申请号: US13488628申请日: 2012-06-05
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公开(公告)号: US20130045492A1公开(公告)日: 2013-02-21
- 发明人: Robert Babb , John McWhirter , Lynn MacDonald , Sean Stevens , Samuel Davis , David R. Buckler , Karolina A. Hosiawa , Andrew J. Murphy
- 申请人: Robert Babb , John McWhirter , Lynn MacDonald , Sean Stevens , Samuel Davis , David R. Buckler , Karolina A. Hosiawa , Andrew J. Murphy
- 申请人地址: US NY Tarrytown
- 专利权人: Regeneron Pharmaceuticals, Inc.
- 当前专利权人: Regeneron Pharmaceuticals, Inc.
- 当前专利权人地址: US NY Tarrytown
- 主分类号: C12P21/00
- IPC分类号: C12P21/00 ; G01N21/64 ; G01N33/566
摘要:
A genetically modified mouse is provided, wherein the mouse expresses an immunoglobulin light chain repertoire characterized by a limited number of light chain variable domains. Mice are provided that express just one or a few immunoglobulin light chain variable domains from a limited repertoire in their germline. Methods for making bispecific antibodies having universal light chains using mice as described herein, including human light chain variable regions, are provided. Methods for making human variable regions suitable for use in multispecific binding proteins, e.g., bispecific antibodies, and host cells are provided. Bispecific antibodies capable of binding first and second antigens are provided, wherein the first and second antigens are separate epitopes of a single protein or separate epitopes on two different proteins are provided.
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