发明申请
US20130065296A1 BACULOVIRUSES WITH ENHANCED VIRION PRODUCTION AND A METHOD FOR THE PRODUCTION OF BACULOVIRUSES
有权
具有增强的病毒生产的细菌病毒和生产细菌病毒的方法
- 专利标题： BACULOVIRUSES WITH ENHANCED VIRION PRODUCTION AND A METHOD FOR THE PRODUCTION OF BACULOVIRUSES
- 专利标题（中）： 具有增强的病毒生产的细菌病毒和生产细菌病毒的方法
-
申请号： US13231324申请日： 2011-09-13
-
公开(公告)号： US20130065296A1公开(公告)日： 2013-03-14
- 发明人: Paul McCray , Patrick Sinn , Gary Blissard
- 申请人： Paul McCray , Patrick Sinn , Gary Blissard
- 申请人地址： US IA Iowa City US NY Ithaca
- 专利权人： UNIVERSITY OF IOWA RESEARCH FOUNDATION,BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH
- 当前专利权人： UNIVERSITY OF IOWA RESEARCH FOUNDATION,BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH
- 当前专利权人地址： US IA Iowa City US NY Ithaca
- 主分类号： C12N7/00
- IPC分类号： C12N7/00
摘要：
A method of co-expressing a portion of the VSV G protein gene or a truncated “stem” portion with GP64 and a retrovirus increases the titer of retroviral vectors. A truncated VSV G protein, preferably comprised of a small segment from the C-terminal portion of the ectodomain plus the transmembrane (TM) and cytoplasmic tail (CTD) domains of VSV G, co-expressed with retroviral vectors, enhances the production titers of the retroviral vectors. A preferred embodiment uses a VSV G construct that includes an N-terminal c-Myc epitope plus 42 amino acids from the C-terminal portion of the ectodomain, 20 amino acids from the predicted TM domain, and 29 amino acids from the predicted CTD of the VSV G protein.