发明申请
US20130078661A1 Compounds and Methods for FRET Based Measurement of Enzyme Activity
审中-公开
基于FRET测定酶活性的化合物和方法
- 专利标题： Compounds and Methods for FRET Based Measurement of Enzyme Activity
- 专利标题（中）： 基于FRET测定酶活性的化合物和方法
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申请号： US13701930申请日： 2011-06-06
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公开(公告)号： US20130078661A1公开(公告)日： 2013-03-28
- 发明人: Sriram Neelamegham , Kannayakanahalli Dayananda
- 申请人： Sriram Neelamegham , Kannayakanahalli Dayananda
- 申请人地址： US NY Amherst
- 专利权人： The Research Foundation for The State University of New York
- 当前专利权人： The Research Foundation for The State University of New York
- 当前专利权人地址： US NY Amherst
- 国际申请： PCT/US2011/039232 WO 20110606
- 主分类号： C07K14/755
- IPC分类号： C07K14/755 ; C12Q1/37
摘要：
Provided are compositions and methods for Fluorescence/Forster Resonance Energy Transfer (FRET) analysis of cleavage of Von Willebrand Factor (VWF) polypeptides by the metalloprotease ADAMTS13. The polypeptides contain: i) a first fluorescent protein ii) a VWF amino acid sequence and iii) a second fluorescent protein. The VWF amino acid can contain any of a variety of amino acid substitutions. The method involves contacting a sample that contains ADAMTS13 and using FRET based measurements to determine ADAMTS13 cleavage of the recombinant polypeptide.
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