发明申请
US20130130313A1 METHOD OF SYNTHESIZING A SUPPRESSOR tRNA, DNA CONSTRUCT AND USE THEREOF FOR PRODUCING A NON-NATURAL AMINO ACID-INCORPORATED PROTEIN
有权
合成抑制剂tRNA的方法，DNA构建及其用于生产非天然氨基酸合成蛋白的方法
- 专利标题： METHOD OF SYNTHESIZING A SUPPRESSOR tRNA, DNA CONSTRUCT AND USE THEREOF FOR PRODUCING A NON-NATURAL AMINO ACID-INCORPORATED PROTEIN
- 专利标题（中）： 合成抑制剂tRNA的方法，DNA构建及其用于生产非天然氨基酸合成蛋白的方法
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申请号： US13460773申请日： 2012-04-30
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公开(公告)号： US20130130313A1公开(公告)日： 2013-05-23
- 发明人: Shigeyuki YOKOYAMA , Kensaku Sakamoto , Nobumasa Hino , Takahito Mukai , Takatsugu Kobayashi
- 申请人： Shigeyuki YOKOYAMA , Kensaku Sakamoto , Nobumasa Hino , Takahito Mukai , Takatsugu Kobayashi
- 申请人地址： JP Wako-shi
- 专利权人： RIKEN
- 当前专利权人： RIKEN
- 当前专利权人地址： JP Wako-shi
- 优先权： JP2006-045788 20060222
- 主分类号： C12N15/85
- IPC分类号： C12N15/85 ; C12P21/00
摘要：
There are provided a DNA construct comprising a suppressor tRNA gene of a non-eukaryote containing no internal promoter functioning in a eukaryotic cell, and a eukaryotic or bacteriophage promoter linked at the 5′ end of the tRNA gene, a method for synthesizing a suppressor tRNA by using the DNA construct, and a process for producing protein incorporating a non-natural amino acid by using the same.