发明申请
- 专利标题： BIOLOGICAL SUBSTANCE DETECTION METHOD
- 专利标题（中）： 生物物质检测方法
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申请号： US13819453申请日： 2011-08-30
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公开(公告)号： US20130157287A1公开(公告)日： 2013-06-20
- 发明人: Kensaku Takanashi , Hideki Gouda , Hisatake Okada , Yasushi Nakano , Kohsuke Gonda , Motohiro Takeda , Noriaki Ohuchi
- 申请人： Kensaku Takanashi , Hideki Gouda , Hisatake Okada , Yasushi Nakano , Kohsuke Gonda , Motohiro Takeda , Noriaki Ohuchi
- 申请人地址： JP Sendai-shi, Miyagi JP Hino-shi, Tokyo
- 专利权人： TOHOKU UNIVERSITY,KONICA MINOLTA MEDICAL & GRAPHIC, INC.
- 当前专利权人： TOHOKU UNIVERSITY,KONICA MINOLTA MEDICAL & GRAPHIC, INC.
- 当前专利权人地址： JP Sendai-shi, Miyagi JP Hino-shi, Tokyo
- 优先权： JP2010-193154 20100831
- 国际申请： PCT/JP2011/069553 WO 20110830
- 主分类号： G01N21/64
- IPC分类号： G01N21/64
摘要：
A biological substance detection method for detecting a biological substance specifically in a pathological specimen, comprising a step of immunologically staining the pathological specimen using a fluorescent label, a step of staining the pathological specimen with a staining reagent for morphology observation purposes (eosin) to observe the morphology of the pathological specimen, a step of irradiating the stained pathological specimen, with excited light to cause the emission of a fluorescent and detecting the biological substance in the pathological specimen. In the step of immunologically staining the pathological specimen, a special fluorescent particle for which the excitation wavelength appears in a region that is different from the excitation wavelength region of eosin is used as the fluorescent label.
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